Extracellular Production of Amylase and Protease by Penicillium Purpurogenum BKS9

Abstract

In the present study, Penicillium purpurogenum BKS9 was used with different agro waste substrates i.e. starch, wheat bran, soya powder, boiled rice, unboiled rice and milk powder for the production of both amylase and protease by liquid static surface fermentation (LSSF). Among the various substrates tested, wheat bran (WB) was found to be the best substrate for maximum (112.64 U/ml) amylase production whereas soya powder (121.23 U/ml) for production of protease. Immobilization study also revealed that the highest amylase was observed (137.6 U/ml) when wheat bran was used as substrate whereas maximum protease production (130.73 U/ml) with soya seed powder. Maximum biomass production was observed (4.4 ± 0.2 g/50ml) when unboiled rice was taken as a substrate in comparison to other substrate.