Gene inactivation study on gntK, a putative C-methyltransferase gene in gentamicin biosynthesis from Micromonospora echinospora

Abstract

GntK harbors methyltransferase-related cobalaminbinding domain and radical S-adenosylmethionine domain. The gntK-inactivation mutant of Micromonospora echinospora accumulated higher levels of gentamicin Cla and lower levels of gentamicin C1 and C2 isomers compared to the wild-type strain. Based on these results, we propose that GntK is involved in C-methylation on C-6' in gentamicin X2 but is dispensable in gentamicin biosynthesis. © The Korean Society for Applied Biological Chemistry and Springer 2012.