Evaluation of Anticancer Activities of Crude Extracts of Apium graveolens L. Seeds in Two Cell Lines, RD and L20B in vitro

Abstract

Present study was designed to evaluate anticancer effect of Apium graveolens seed extracts (aqueous, ethanolic and hexane extracts) in vitro on two cell lines (RD and L20B) using different concentrations of A. graveolens seed extracts (6.25, 12.5, 25, 50, 100 and 200 µg/ml) for an incubation period of 48 hours. The results revealed a clear cytotoxic activity of those extracts on growth of RD cancer cell line, and the effect was concentration-dependent. The results also, suggested that the hexane extract of A. graveolens showed the best cytotoxic activity on RD cell line, especially at the concentrations 100 and 200 µg/ml. In contrast, there was no significant cytotoxic effect of the extracts on the L20B transformed cells, with the exception of hexane extract at the concentration 200 µg/ml, in which a significant growth inhibitory effects were observed.