The Effects of 528 Hz Sound Wave to Reduce Cell Death in Human Astrocyte Primary Cell Culture Treated with Ethanol

Abstract

Objective: Alcohol consumption, because of damaging effects on various tissues including nerves system, causes a major problem in human societies. Nowadays, Usages of the non-pharmacological and non-invasive agents is common. Sound waves which are classified in non-invasive agent, beside on stimulation the auditory cells, also impact on non- auditory cells. It has been reported that the frequency of 528 HZ is related to the note MI and it shown some strange effects such as increasing the ability of DNA repair. Materials and methods: In this paper, with MTT, LDH and ROS assays, it was evaluated the effect of ethanol on astrocytes primary cell culture which exposed to this frequency. Results: The current results show that in IC50 of ethanol, the frequency of 528 Hz increased cells viability about 20% and the level of ROS production has been reduced up to 100%. Conclusion: Therefore, the use of these sound waves can be useful to reduce the toxic effects of ethanol on astrocytes cells culture.