Analysis of genetic structure in Slovak pinzgau cattle using five candidate genes related to milk production traits

Abstract

The goal of the paper was to identify genetic structure of five candidate genes for milk production in Slovak Pinzgau breed. A total of 86 mothers of bulls of Slovak Pinzgau cattle were use in this study. To genotype of cows for candidate genes we used PCR methods (PCR-RFLP, ARMS-PCR, multiplex PCR-RFLP). On the basis of PCR analyses we established genotype structure of cattle population and calculated allelic frequencies. Effectiveness of allele incidence and genetic diversity was evaluated with following parameters: theoretical heterozygosity (He exp), experimental heterozygosity (He obs), polymorphism information content (PIC), expected homozygosity (E), effective number of alleles (ENA), level of possible variability realization (V%). Slovak Pinzgau cattle exhibit the high values of heterozygosity, polymorphism information content, effective number of alleles and level of possible variability realization for genes CSN2, CSN3 and LALBA. In opposite, for genes CSN1S1 and LGB show high values of homozygosity.