Low Temperature Enzymatic Hydrolysis (LTEH) and Fermentation for Bioethanol Generation from Suweg (Amorphophallus campanulatus B) Starch

Abstract

The ethanol generation through low temperature enzymatic hydrolysis (LTEH) of Suweg (Amorphophallus campanulatus B) starch followed by anaerobic fermentation using Saccharomyces cerevisiaehas been studied. Granular starch hydrolyzing enzyme (GSHE) as Stargen TM 002 was used in the hydrolysis to degrade starch into reducing sugar at 30°C and pH 4. The concentration of Suweg starch was 200 g/L, concentration of enzyme were 1; 1.5 and 2% (w/w), respectively. The fermentation was carried out at pH 4.5 and 30 °C for 72 h employing yeast concentration of 1gL-1. The ideal state of the procedure was fermentation utilizing concentration of Suweg starch 200 g L-1, concentration enzyme 1.5% (w/w), pH 4.5 and 30°C, for 60 h, which came about reducing sugar grouping of 31.32 g/L and further used for ethanol generation. It was discovered that most extreme ethanol fixation and profitability 13.12 g L-1 and 0.3727 gL-1h-1, separately. Although the result shows that Suweg starch is a potential raw material for ethanol generation, further investigations are required in both generation sustainability and techno-economical considerations.