Presence of luteinizing hormone-releasing hormone binding sites in cultured porcine lymphocytes

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Abstract

Membrane-enriched homogenates of fresh and cultured (48 h) porcine lymphocytes were assayed for the presence of specific LHRH receptors by saturation and displacement analysis using [D-Ser-(TBU)6, des-GLY-NH210] LHRH-EA as the labeled and unlabeled ligand. Membrane-enriched homogenates of porcine pituitaries served as positive controls while porcine granulosa cell membranes and crude liver homogenates served as negative controls. Specific high-affinity LHRH receptors were found in porcine pituitaries (K(d) = 0.3 nM) and cultured lymphocytes (K(d) = 13 nM) but not in fresh lymphocytes. No specific binding was observed in negative control tissues. Porcine lymphocytes have measurable high-affinity LHRH receptors after 48 h of culture.

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Standaert, F. E., Chew, B. P., De Avila, D., & Reeves, J. J. (1992). Presence of luteinizing hormone-releasing hormone binding sites in cultured porcine lymphocytes. Biology of Reproduction, 46(6), 997–1000. https://doi.org/10.1095/biolreprod46.6.997

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