Indirect spectrophotometric method for the determination of bisacodyl in commercial dosage forms and in environmental water samples

Abstract

Objective: A simple, sensitive, and accurate indirect spectrophotometric method for the determination of bisacodyl in pure form and in some of its pharmaceutical preparations. Method: The method is based on the oxidation of bisacodyl by iron (III), and subsequent complexation of iron (II) with o-phenanthroline. Results: Forming a red-colored complex (ferroin) having the maximum absorbance at 510 nm. Beer's law is obeyed in the concentration range of 0.5-5 µg/ml. The molar absorptivity and sandell's sensitivity were 1.55×10 4 L.mol-1 .cm-1 and 0.0233µg.cm-2 respectively. The relative standard deviation (RSD) was less than 1.5 (n=11). The limits of detection and quantitation are 0.083 and 0.25 µg.ml-1 respectively. Conclusion: The method is applied successfully for determination of bisacodyl in environmental water samples and in some pharmaceutical formulations (tablets and suppositories). A statistical comparison of these results with those of official method shows good agreement and indicates no significant difference in the precision. ‫اﻟﺨﻼﺻﺔ‬ ‫اﻟﻬﺪف‬ : ‫ﺑﻌﺾ‬ ‫وﻓﻲ‬ ‫اﻟﻨﻘﻴﺔ‬ ‫ﺣﺎﻟﺘﻪ‬ ‫ﻓﻲ‬ ‫اﻟﺒﺴﻜﻮداﻳﻞ‬ ‫ﻟﺘﻘﺪﻳﺮ‬ ‫ﻋﺎﻟﻴﺔ‬ ‫دﻗﺔ‬ ‫وذات‬ ‫وﺣﺴﺎﺳﺔ‬ ‫ﺑﺴﻴﻄﺔ‬ ‫ﻃﺮﻳﻘﺔ‬ ‫وﺻﻒ‬ ‫ﻣﺴﺘﺤﻀﺮاﺗ‬ ‫ﻪ‬ ‫اﻟﺼﻴﺪﻻﻧﻴﺔ‬. ‫اﻟﻌﻤﻞ‬ ‫ﻃﺮﻳﻘﺔ‬ : ‫اﻟﺤﺪﻳﺪ‬ ‫اﻟﺘﻔﺎﻋﻞ‬ ‫ﻧﺎﺗﺞ‬ ‫ﻳﻜﻮن‬ ‫ﺣﻴﺚ‬ ‫اﻟﺜﻼﺛﻲ‬ ‫اﻟﺤﺪﻳﺪ‬ ‫ﺑﻮاﺳﻄﺔ‬ ‫اﻟﺒﺴﻜﻮداﻳﻞ‬ ‫أآﺴﺪة‬ ‫ﻋﻠﻰ‬ ‫اﻟﻄﺮﻳﻘﺔ‬ ‫ﺗﻌﺘﻤﺪ‬ ‫ﺑﺪورﻩ‬ ‫واﻟﺬي‬ ‫اﻟﺜﻨﺎﺋﻲ‬ ‫ﻳﺘﻔﺎﻋﻞ‬ ‫او‬ ‫آﺎﺷﻒ‬ ‫ﻣﻊ‬ ‫رﺛﻮﻓﻴﻨﺎﻧﺜﺮوﻟﻴﻦ‬) ‫اﻟﻔﺮوﻳﻦ‬ (‫اﻟﻨﺘﺎﺋﺞ‬ : ‫اﻟﻠﻮن‬ ‫اﺣﻤﺮ‬ ‫ﻣﻌﻘﺪ‬ ‫ﺗﻜﻮﻳﻦ‬ ‫اﻟﻤﻮﺟﻲ‬ ‫اﻟﻄﻮل‬ ‫ﻋﻨﺪ‬ ‫اﻣﺘﺼﺎص‬ ‫اﻗﺼﻰ‬ ‫ﻟﻪ‬ ٥١٠ ‫ﻧﺎﻧﻮﻣﻴﺘﺮ‬. ‫ﻗﺎﻧﻮن‬ ‫ان‬ ‫ﻟﻮﺣﻆ‬ ‫وﻗﺪ‬ ‫ﺑﻴﻦ‬ ‫ﺗﺘﺮاوح‬ ‫اﻟﺘﻲ‬ ‫اﻟﻜﻤﻴﺎت‬ ‫ﻋﻠﻰ‬ ‫ﻳﺴﺮي‬ ‫ﺑﻴﻴﺮ‬ ٠.٥-٥ ‫ﻣﻮﻻرﻳﺔ‬ ‫وﺑﺎﻣﺘﺼﺎﺻﻴﺔ‬ ‫ﺑﺎﻟﻤﻠﻴﻮن‬ ‫ﺟﺰء‬ ١.٥٥ × ٤ ١٠ ‫ﻟﺘﺮ‬. ‫ﻣﻮل‬-١