A urinary Bcl-2 surface acoustic wave biosensor for early ovarian cancer detection

22Citations
Citations of this article
26Readers
Mendeley users who have this article in their library.

Abstract

In this study, the design, fabrication, surface functionalization and experimental characterization of an ultrasonic MEMS biosensor for urinary anti-apoptotic protein B-cell lymphoma 2 (Bcl-2) detection with sub ng/mL sensitivity is presented. It was previously shown that urinary Bcl-2 levels are reliably elevated during early and late stages of ovarian cancer. Our biosensor uses shear horizontal (SH) surface acoustic waves (SAWs) on surface functionalized ST-cut Quartz to quantify the mass loading change by protein adhesion to the delay path. SH-SAWs were generated and received by a pair of micro-fabricated interdigital transducers (IDTs) separated by a judiciously designed delay path. The delay path was surface-functionalized with monoclonal antibodies, ODMS, Protein A/G and Pluronic F127 for optimal Bcl-2 capture with minimal non-specific adsorption. Bcl-2 concentrations were quantified by the resulting resonance frequency shift detected by a custom designed resonator circuit. The target sensitivity for diagnosis and identifying the stage of ovarian cancer was successfully achieved with demonstrated Bcl-2 detection capability of 500 pg/mL. It was also shown that resonance frequency shift increases linearly with increasing Bcl-2 concentration. © 2012 by the authors; licensee MDPI, Basel, Switzerland.

Cite

CITATION STYLE

APA

Onen, O., Sisman, A., Gallant, N. D., Kruk, P., & Guldiken, R. (2012). A urinary Bcl-2 surface acoustic wave biosensor for early ovarian cancer detection. Sensors (Switzerland), 12(6), 7423–7437. https://doi.org/10.3390/s120607423

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free