Molecular detection of antibiotics resistance genes in burkholderia cepecia isolated from diabetic foot infection

Abstract

This study aimed to isolate and diagnose Burkholderia cepetia from clinical specimens of diabetic foot and study the resistance of bacteria to antimicrobial agent in Najaf governorate from Augest 2019 to November 2019, which includes 120 clinical specimens for both sexes with an age ranged between (35-70) years old. The diagnosis of bacteria isolates was based on microscopy, as well as the culture and biochemical characteristics as an initial diagnosis. The final diagnosis by the Vitek-2 compact system .Burkholderia cepacia 8(6.6%). Antibiotic sensitivity test was examined by dick diffution method, Burkholderia cepacia isolates showed high level of resistance almost for all β-lactam antibiotic classes under study which included;, ceftriaxone, cefoxitin and Cefepime, with percentage of (100%); ticarcllin with clavulalnic acide,piperacllin, ceftazidime, tobramycine, ciproflaxcin and levofloxacin with percentage of (87.5%); aztreonam and amikacine with percentage(62.5); meropenem, imipenem and gentiamycin with percentage of (37.5%).At molecular study, the investigated the presence of antibiotic-resistant genes (blaImp, blaOxa, blaKpc,blaCTX-M) using PCR technique and electrophoresis systems. 6/8 Burkholderia cepacia isolates were with blaOXA and (6/8) of isolates carry blaCTX-M gene, all isolates of Burkholderia cepacia gave negative result of blaIMP and blaKPC gene. Finally PCR analysis showed that the integron gene was (3/8 %).