Cellulase Production by Trichoderma viride in Submerged Fermentation using Response Surface Methodology

Abstract

The potential of Trichoderma viride for cellulase production using seed pods of silk cotton tree as substrate in submerged fermentation by response surface methodology has been investigated. Three variables like substrate concentration, peptone and KH2PO4 were optimized at three levels. The optimum carboxymethylcellulase (CMCase) activity and filter paper cellulase (FPase) activity was obtained after 96 hours of incubation with optimum conditions of media containing 5% substrate concentration, 0.05% peptone and KH2PO4 concentration of 0.5% with pH 5.0 at incubation temperature of 30ᵒC. The model proposed was found significant. The cellulase produced could be potentially used in industries especially for biofuel production.