PHYTOCHEMICAL INVESTIGATION OF ELEUSINE INDICA FOR IN-VIVO DIURETIC AND IN-VITRO ANTI-UROLITHIATIC ACTIVITY

Abstract

Eleusine indica L. Gaertn also known as wire grass belonging to family poaceae. The plant is used for deworming, eliminate or reduce cough and lung troubles, dysentery, heart problems and high blood pressure, spleen and liver complaints, bladder and kidney stone and in case of sprains, dislocation of bones and lumbago. The plant contains cyanogenetic glucoside, albuminoids, starch, fatty oils, phenolic compounds and flavonoids. The present investigation was carried out to isolate, purify and characterize active constituents from the whole plant of Eleusine indica L. Gaertn. Powdered material of whole plant of Eleusine indica L. Gaertn was taken for the preparation of extracts using soxhlet apparatus. The various phytochemical tests were performed for alkaloids, glycosides, phenolic, flavonoids etc. TLC and HPTLC were done for isolation of compounds. The isolated compounds were further characterized by FTIR, Ultraviolet spectroscopy and GC-MS. The diuretic activity was evaluated by using in-vivo model and anti-urolithiatic activity was evaluated by using in-vitro model. In case of diuretic activity ethanol extract increase the total urine volume and total content of sodium, potassium and chloride ions in urine. In the case of antiurolithiatic activity ethanolic extract significantly dissolved the calcium oxalate stones. Thus ethanolic extract of whole plant of Eleusine indica L. Gaertn have significant diuretic and anti-urolithiatic activity.