A Multiplex-Urease PCR Assay for Detection of Helicobacter pylori Infection Directly from Gastric Biopsy Specimens and Comparison of Multiplex- Urease PCR Results with Rapid Urease Test and Histopathology

Abstract

See, stats, and : https : / / www . researchgate. net / publication / 316904393 A - Urease Helicobacter Gastric . . . Article DOI : 10 . 4172 / 2327 - 5073 . 1000279 CITATIONS 0 READS 15 10 , including : Some : H2020 - MSCA - RISE - Advanced novel : implications . METABLE View Design , Synthesis , and – Thiourea Antituberculosis Nurdan Acibadem 87 SEE Tanil Acibadem 89 , 080 SEE O . Uğur Acibadem 147 SEE All . The . All - text and , letting . Abstract Purpose : To develop a multiplex - urease PCR assay specific for ureA and ureB genes directly from gastric biopsy specimens and compare the results of multiplex - urease PCR assay with rapid urease test (RUT) and histopathology . Methodology : This study was conducted on 109 patients . A RUT was run ; histopathological staining and multiplex urease PCR were applied to biopsy specimen to detect H . pylori . Multiplex - urease PCR , RUT and histopathology results were compared by calculating Cohen ' s kappa coefficient . Results : A multiplex - urease PCR assay specific for ureA and ureB genes was developed to detect H . pylori directly from biopsy specimens . Cohen ' s kappa coefficient results of histopathological staining and multiplex - urease PCR indicate a substantial agreement . There was a moderate agreement between the results of histopathological staining and RUT results . There is a fair agreement between the multiplex urease PCR and the RUT results . Furthermore , the multiplex - urease PCR can detect H . pylori in some samples that are identified as negative by the rapid urease test and histopathological staining method . Moreover , in some patient samples ureA could not be detected while ureB detected . Conclusion : Multiplex - urease PCR assay was developed to detect ureA and ureB genes of H . pylori directly from gastric biopsy specimens . Comparison results indicated that detection rate of H . pylori with multiplex - urease PCR and histopathological staining is higher than the RUT . Moreover , it is critical to apply RUT test to biopsy specimens taken from both antrum and corpus part as in multiplex urease PCR assay . Furthermore , developing a multiplex PCR for both ureA and ureB is essential to detect active H . pylori infection .