The relationship between the intracellular redox status of immune cells and progression of hepatitis C virus related chronic liver disease

Abstract

It has been suggested that oxidative stress participates in the pathogenesis of hepatitis C virus infection. It also has been made clear that redox status in T cell and macrophage relatesto the activity of virus infectious disease such as HIV infection. With such background we evaluated the relationship between the intracellular redox status of T cell and macrophage and the activity of HCV positive chronic liver disease. Intracellular GSH and GSSG levels of T cell and macrophage were determined in twenty-five HCV positive asymptomatic carriers (C-ASC), sixty-three chronic hepatitis patients (C-CH), ten HCV positive liver cirrhosis patients (C-LC) and twenty-nine healthy controls. The intracellular GSH levelsof T cell (T-GSH) significantly decreased in both C-CH and C-LC compared with healthy controls. No significant differencesin the T-GSH levelswere found between healthy controlsand C-ASC. T-GSH levels of C-CH and C-LC were significantly lower compared with C-ASC. The intracellular GSH levelsof macrophage (CD14-GSH) of C-LC were significantly decreased compared with healthy controls. The CD14-GSH levelsof C-CH and C-LC were significantly lower compared with C-ASC. There wasno correlation between intracellular GSH, GSSG levels and the serum levels of iron-related markers, fibrogenesis markers and other clinical parameters. These results suggest that the intracellular redox status of T cell and macrophage relates to the progression of HCV related chronic liver disease. © 2004, The Japan Society for Clinical Immunology. All rights reserved.