Rapid determination of membrane transport parameters in adherent cells

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Abstract

Reported here is a new method that permits rapid (approximately 5 s) determinations of membrane transport phenomena in cells grown in monolayers at the base of 17-mm glass scintillation vials. The method is convenient, cost effective and requires no special apparatus. Initial uptake rates, steady-state and free substrate levels are demonstrated in ZR-75-1 breast cancer and Chinese hamster ovary cell lines using methotrexate, a model agent transported by the reduced folate carrier. The technique should be applicable to the study of the transport properties in a broad range of substrates and cells in monolayer culture.

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APA

Sharif, K. A., & Goldman, I. D. (2000). Rapid determination of membrane transport parameters in adherent cells. BioTechniques, 28(5), 926–932. https://doi.org/10.2144/00285st06

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