Development of a one-step analysis method for several amino acids using a microfluidic paper-based analytical device

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Abstract

A one-step analysis method was developed for four types of amino acids using a microfluidic paper-based analytical device fabricated from chromatography filtration paper and laminate films. Aminoacyl-tRNA synthetase was used to detect each amino acid. The obtained laminated paper-based analytical device (LPAD) contained four enzymatic reaction areas. Colorimetric detection was performed based on the molybdenum blue reaction. A model method for the simple, easy, and simultaneous detection of several amino acid concentrations was suggested, in contrast to the conventional methods such as HPLC or LC–MS. The method provided a selective quantification at the ranges of 3.6–100 μM for tryptophan, 10.1–100 μM for glycine, 5.9–100 μM for histidine and 5.6–100 μM for lysine with a detection limit of 1.1 μM, 3.3 μM, 1.9 μM and 1.8 μM, respectively. LPAD fabrication was considerably simple, and the subsequent detection process was easy and required a short period of time (within 15 min).

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Kugimiya, A., Wakimoto, S., Kohda, J., Nakano, Y., & Takano, Y. (2022). Development of a one-step analysis method for several amino acids using a microfluidic paper-based analytical device. Scientific Reports, 12(1). https://doi.org/10.1038/s41598-022-07408-9

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