Biosynthesis of CuO NPs and its anticancer activity on human colon cancer cell lines (HT-29)

Abstract

The environmentally friendly syntheses of the nanoparticles through the green way from the extracts of the plants has proven itself in the field of modern sciences, with improved drug efficiency and less toxicity. The study groups in the have bio-synthesized cost-effective and stable copper oxide nano-particles (CuONPs) from plant leaves (i.e. the Turmeric). A variety of the analytical approaches, like the UV-Visible Spectroscopy (UV-Vis), Fourier-Transformation Infra-red Spectroscopy (FTIR), Scanning Electron Microscopy (SEM) and X-Ray Diffraction (XRD), have been utilized for the confirmation of the syntheses of the crystalline CuONPs from the extract of the turmeric leaves (SEM), Transmission Electron Microscopy (TEM) and Selected Area Electron Diffraction (SAED) pattern. Synthesized CuONPs have been tested as well for the anti-cancer activity with the use of the MTT (3- (4,5dimethyl-2tiazolyl)-2,5-diphenyl-2tetrazolium bromide) assay on the cell lines of the human colon cancer (HT29). The results have shown that the synthesized CuONPs had a high anti-cancer cytotoxicity on the cell lines of the human colon cancer (HT-29) with an IC-50 value of 40 g mL-1, which was briefly addressed in the present paper.