Major Components of Clinically used Bee Venom Pharmacopuncture ※

Abstract

[Abstract] Objectives : Thepurposeofthisstudywastoanalyzethecomponentsoftheclinicallyusedbee venom(BV)pharmacopuncture. Methods : Twokindsofbeevenompharmacopuncture(BV-IandII),threekindsofseparatepu-rificationBV(SPBV)pharmacopuncture(SPBV-I,II,andIII),andapitoxinwereinvestigatedin thisstudy.Weperformedacomponentanalysisofmelittin,apamin,andphospholipaseA 2 usinghigh-performanceliquidchromatography(HPLC). Results : 1.BV-Icontainedapproximately40%moremelittinthanBV-IIdid. 2.InthethreeseparatepurificationBVpharmacopuncture,SPBV-I,SPBV-II,andSPBV-III, phospholipaseA 2 contentdecreasedremarkably. 3.ThemelittincontentinSPBV-Iincreasedby5%comparedtothatinBV-I. 4.TheamountofmelittininapitoxinwassimilartothatinSPBV-I. ThisisanOpen-AccessarticledistributedunderthetermsoftheCreativeCommonsAttributionNon-CommercialLicense(http://creativecommons.org/licenses/by-nc/3.0)whichpermitsunrestrictednon-commercialuse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperlycited. TheAcupuncture istheJournalofKoreanAcupuncture&MoxibustionMedicineSociety.(http://www.TheAcupuncture.org) Copyright2017KAMMS.KoreanAcupuncture&MoxibustionMedicineSociety.Allrightsreserved.