cAMP-related second messenger pathways modulate hearing function in Aedes aegypti mosquitoes

Abstract

The powerful ears of male Aedes aegypti mosquitoes facilitate identification and localization of mating partners via detection of female flight tones. Male hearing function is modulated by the efferent release of neurotransmitters, though the secondary mechanisms underlying this modulation remain unclear. Here, we investigated these mechanisms using octopamine as a model, as octopamine modulates hearing function and the erection status of fibrillar hairs lining male ears. We found that pharmacological interference with octopamine receptors alters hearing function at multiple levels and identified the second messenger cAMP as likely mediating these changes. Furthermore, the erection status of male ear fibrillar hairs could be altered by targeting specific sub-types of octopamine receptors, but these changes were not linked to changes in ear frequency tuning. Finally, we suggest that octopamine α2 receptors linked to fibrillar hair erection may not always produce functional proteins across species, with downstream implications for hearing behaviors.