Anti-metastatis Profile of Boesenbergia Pandurata towards MCF-7/HER2 Cells

Abstract

The development of cancer at an advanced stage is signed with metastasis that trigger the high of mortality caused breast cancer, possitive HER2 type mainly. Boesenbergia pandurata known as medicinal plant possessing anticancer potential due to the cytototoxic and antimetastatic characteristic of its active compound. The aim of this study is to observe the inhibitory of migration of Boesenbergia pandurata ethanolic extract (BPEE) in combination with doxorubicin (Dox) on MCF-7/HER2 cells. BPEE was prepared by 96% ethanol maceration. Under MTT assay, BPEE decreased the cells viability with IC50 value of 23 ± 3.9 μg/mL. Lamellipodia and wound healing assay analysis showed that BPEE (5 μg/mL) and its combination with doxorubicin (10 nM) inhibited cells migration after 48 hours observation, while gelatin zymography analysis showed that this combination did not affect the expression of MMP2 and MMP9 proteins, but single treatment of BPEE was more able to decrease the expression of both MMP. The combination with those concentrations inhibited the cells migration but not with the cells viability. Thus, BPEE is potential to be developed as antimetastatic agent. The mechanism itself needs to be explored further.