Development of spectrophotometric method for estimating clonazepam in its pure form and in pharmaceutical tablets

Abstract

A study aimed to recommend spectrophotometric method for the determination of clonazepam (CLZ.) in pure form and their pharmaceutical tablets by the reaction of sited drug with ferric chloride in presence potassium ferric cyanide in acid medium to form prussian blue complex and determine it by UV-Vis spectrophotometric at 526 nm. The variables that affect the completion of reaction have been carefully optimized. The linearity range was (2-35μg. mL-1) with a molar absorptivity of 2.8035×104 L.mol-1. cm-1. The limit of detection was 0.5703μg. mL-1 and Sandell's sensitivity value was 0.0103μg. cm-2. The suggest method was successfully used to estimate clonazepam in commercial formulations.