Analytical Characteristics of GC/MS and HPLC according to the Concentration Distribution of PAHs

Abstract

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License(http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. 한국산업보건학회지, 제25권 제3호(2015) ABSTRACT Objectives: The purpose of this study was to determine the best method to analyze PAHs at extremely low concentrations. To this end, 16 PAHswere analyzed simultaneously by GC/MS, HPLC/FLD and HPLC/UVD, and the analytical characteristics of HPLC and GC/MS were compared. Methods: This study was conducted by GC/MS and HPLC/FLD/UVD, and evaluated linearity, precision and detection limit. Standard solutions were prepared for 21 samples in the range of 0.00001~1.0 ㎍/mL and the samples were divided into four groups. All samples were made in three sets and analysis was replicated seven times. Results: Sixteen PAHs could be simultaneously separated by HPLC and GC/MS, and the adequate equipment was HPLC/FLD. The retention times by HPLC were shorter than GC/MS, and HPLC had better separation for most PAHs than GC/MS. The peaks of naphthalene and naphthalene-D8 partially overlapped for GC/MS. HPLC/FLD had a 20-2000 times lower limit of detection than GC/MS and UVD. However FLD was not adequate for analyzing acenaphthylene because it has too low a fluorescence quantum yield to be detected. The precision of HPLC/FLD/UVD and GC/MS showed less than 20% at 0.001 ㎍/mL PAHs and when the concentration was higher, the coefficient of variation was decreased. HPLC/FLD was better for the overall detection of limits. Conclusions: The results indicate that the HPLC/FLD method has good linear range, precision and a detection of limits from 0.00001~0.0001 ㎍/mL for all 16 PAHs. This study contributes to providing useful data for analysis technology and can be applied to occupational exposure measurement for PAHs in workplaces. I. 서 론 다환방향족탄화수소(Polycyclic aromatic hydrocarbons, 이하 PAHs)는 2개 이상의 벤젠고리가 선형으로 각을 지어 있거나 밀집된 구조로 이루어져 있는 유기화합물로 화학연료나 유기물의 불완전 연소시 부산물로 발생하는 물질이다(Raiyani et al. 1993). 공기역학적 직경이 2.5 ㎛ 미만의 입자상, 가스상 물질로서 공기 중에 방출되며, 비점과 융점이 높으나 증기압이 낮고, 분자량 증가에 따라 극히 낮은 수용해도를 나타내는 것이 일반적인 성질이다(EPA, 1997). PAHs는 동종 화합물이 수백여 종에 이르며 현재 까지 독성이 알려진 화합물은 benzo[a]pyrene 외 50 여종으로 특히 일부 물질은 유전독성과 발암성을 나 타내는 것으로 알려져 있다(ATSDR, 1995). IARC