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Multiplex high-resolution melting analysis as a diagnostic tool for detection of plasmid-mediated AmpC β-lactamase genes

Journal of Clinical Microbiology (2014) 52(4) 1262-1265
DOI: 10.1128/JCM.00214-14
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Abstract

High-resolution melting (HRM) analysis can be a diagnostic tool to evaluate the presence of resistance genes with the added bonus of discriminating sequence modifications. A real-time, multiplex PCR assay using HRM was designed for the detection of plasmid-mediated ampC genes. The specificity and sensitivity of this assay were 96% and 100%, respectively. Copyright © 2014, American Society for Microbiology. All Rights Reserved.

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APA

Geyer, C. N., & Hanson, N. D. (2014). Multiplex high-resolution melting analysis as a diagnostic tool for detection of plasmid-mediated AmpC β-lactamase genes. Journal of Clinical Microbiology, 52(4), 1262–1265. https://doi.org/10.1128/JCM.00214-14

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