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Determination of natural moisturizing factors in the skin: Raman microspectroscopy versus HPLC

Biomarkers (2017) 22(6) 502-507
DOI: 10.1080/1354750X.2016.1256428
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Abstract

Background: Natural moisturizing factor (NMF) is used as genotypic and phenotypic biomarker in diagnostics. This study is a side-to-side comparison of two different methods to determine NMF in atopic dermatitis patients: Raman microspectroscopy and stratum corneum tape stripping followed by HPLC. Results: Measured NMF values were significantly correlated (R2 =.61; p

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Koppes, S. A., Kemperman, P., Van Tilburg, I., Calkoen-Kwa, F., Engebretsen, K. A., Puppels, G. J., … Kezic, S. (2017). Determination of natural moisturizing factors in the skin: Raman microspectroscopy versus HPLC. Biomarkers, 22(6), 502–507. https://doi.org/10.1080/1354750X.2016.1256428

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