Maltase, Sucrase and .ALPHA.-Amylase Inhibitory Activity of Morus Leaves Extract

Abstract

The composition of hot water extract of morus (Morus alba L.) leaves harvested in China were investigated by HPLC system with photo diode array detector. Chlorogenic acid, 1- deoxynojirimycin, rutin, isoquercitrin, astragalin, caffeic acid, quercetin, quercitrin were identified by comparing with authentic samples. Chlorogenic acid was major component and its content was 1.17%. Molus leaves extract showed stronger maltase and sucrase inhibitory activity than α- amylase inhibitory activity. Chlorogenic acid exhibited relatively strong α- amylase inhibitory activity. Chlorogenic acid played an important role in the overall α- amylase inhibitory activity of extract. 1- Deoxynojirimycin showed the strongest maltase and sucrase inhibitor among the compounds tested. Maltase and sucrase inhibitory activity of morus leaves extract depended largely on 1- deoxynojirimycin. Although the clinical value of morus leaves extract needs to be further evaluated, these results suggest that morus leaves extract might be useful for prevention of diabetes.