Isolation and Purification of Antibacterial Peptide from Bacillus safensis, Endophytica Bacteria from Anthocephalus kadamba

Abstract

The endophytic bacteria is refers to as interior colonization of plants. Endophytic bacteria or fungi colonize the host tissue internally, sometimes in high numbers, without damaging the host or eliciting symptoms of plant disease according to a widely used. In recent work for the isolation and characterization of endophytic bacteria the medicinal trees was selected from Nakshatra Udyan. There were number of reports of the medicinal components present in the plants being the metabolic products of endophytic bacteria. Hence, the nakshatra trees can be the potent source of these metabolites which is having beneficial role for all living system. Accordance to this it was hypothesized that the endophytic bacteria isolated from the nakshatra tress were source for the antimicrobial peptides, biocontrol agent, plant growth promoting hormone. For the isolation of endophytic bacteria the medicinal trees Anthocephalus kadamba was selected. The isolation of endophytic bacteria was carried out from leaf of plant. Identification of endophytic bacteria was done by microbiological and biochemical aspects observing microscopic characteristics and biochemical characteristics by using Bergey’s manual of Determinative and Systematic. The Bacillus sp. was prominent in this isolation. The isolates were identified at molecular level by using the 16 s r DNA technology. The phylogenetic analysis was done by using bioinformatics tools. The isolates were screened for antimicrobial peptide and characterization of these metabolites was done by using Fast Protein Liquid Chromatography.