Physico-chemical characterization and antibacterial activity of a controlled collagenhydroxyapatite-ciprofloxacin release system

Abstract

The aim of our study was to synthesize a biocomposite material using a natural polymer (collagen), hydroxyapatite and ciprofloxacin. We used Fourier-transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), high performance liquid chromatography (HPLC-DAD) and scanning electron microscopy (SEM) for the physico-chemical characterization and we determined its antibacterial activity against Staphylococcus aureus Rosenbach (S. aureus), Pseudomonas aeruginosa (Schroter) Migula (P. aeruginosa), Klebsiella pneumonia (Schroter) Trevisan (K. pneumoniae) and Escherichia coli (Migula) Castellani and Chalmers (E. coli). The release profile of the ciprofloxacin chemisorbed in the material was also studied, and we found that the release was sustained for 30 days.