Bacoside a, withanolide a, and shatavarin IV are beneficial ulcer and ulcer healing actIVity by modulating inflammatory mediators in rats

Abstract

In addition to providing effective antiulcer medicines, their suboptimum safety and efficacy also stirs the search for alternative therapies. Repositioning of drugs is an attractive, convenient, and low risk strategy. The Phytoconstituents Bacoside A, Withanolide A and shatavarin IV obtained from the medicinal plants. The purpose of this analysis was to look at the mechanism of action underling the ulcer protective and ulcer healing capability of these phytoconstituents. The acute toxicity experiment was performed to look for the toxicity of the phytoconstituents, gastro protective effect determined using pylorus ligation induced ulcer model, connection of NO, SH and PGE2 were also tested. Peptic ulcer induced by acetic acid model, Western blotting assay (COX-1 and COX-2) was also performed to elucidate the mechanism of action of Phytoconstituents. No mortality was detected at given dosage in the acute toxicity study. In ulcer protective activity the phytoconstituents, Bacoside A and Shatavarin IV at the dose of 200µg/ml/kg, Withanolide A at the dose of 20µg/ml/kg appreciably reduced the ulcer index and ulcer severity in pylorus ligation induced ulcer model, the antiulcer ability involves several mechanisms like, non participation of NO and SH compounds. Improvement of PGE2 range and the up regulation of COX-2 seems to be directly related to the phytoconstituents antisecretory, ulcer-protective effects. These findings accentuate the gastro protective and gastric healing potency of the Phytoconstituents were mediated via concerted multi-pronged actions, including promotion of gastric protection, augmentation of PGE2, up regulation of COX-II.