A simple method for staining and counting isolated adipose tissue fat cells

Abstract

In similar tests with isolated fat cells from various sources, the total content of triglyceride, in the same way as the amount of DNA which is contained, does not lend itself for use as a basis for comparison. If the number of cells used in the experiment is adopted as a basis for comparison, it is very difficult to distinguish between metabolically active and metabolically inactive cells, just as it is difficult to distinguish between cells and fat droplets. - When stained with acridine orange and a simple cell counting technique used, the fat cells can be counted quickly, and if desired, the count can be repeated with a reasonable degree of accuracy. © 1969 Springer-Verlag.