Effect of calcium and magnesium administration on serum and follicular fluid mineral and protein profiles in buffaloes

Abstract

An attempt was made in buffaloes (7) with calcium (1.86%) magnesium (5%) borogluconate administration (20 ml subcutaneous route) during one complete estrous cycle length to assess the changes in mineral levels in serum and follicular fluid as well as follicular fluid protein profiles. There was no significant change in serum and follicular fluid mineral (Mg, B, Cu) levels. In the follicular fluid proteins, 11 protein spots in treatment group (10.59 to 60.57 kDa, pI3.1 to 9.79) and 6 protein spots in control group (33.43 to 90.91 kDa, pI 4.29 to 9.75) were differentially expressed. External supplementation of calcium and magnesium changes the protein expressions pattern (increased/decreased quantity) in follicular fluid.