Identification of amino acid sequences via X-ray crystallography: A mini review of case studies

Abstract

The sequencing of a protein is a complicated issue. Several methods have been developed in order to establish the amino acid sequences of proteins, including Edman degradation, LC/MS/MS and cDNA sequencing. A number of examples confirm that X-ray crystallography could also be a useful tool for the identification of an amino acid sequence of unknown proteins. Here, we present a short review summarizing the application of crystallography for protein sequence determination and identification.