Sterols from Lindera glauca Blume stem Wood

Abstract

Chipped stem wood from Lindera glauca was extracted repeatedly with 80% aqueous methanol at room temperature, and the concentrated methanolic extract was successively partitioned with ethyl acetate (EtOAc), n-butyl alcohol, and H2O. From the EtOAc fraction, four sterols were isolated through a repeated silica gel and octadecyl silica gel column chromatography. The chemical structures of the sterols were elucidated as β-sitosterol (1), 7-ketositosterol (2), 7β-hydroxysitosterol (3), and daucosterol (4). Among them, compounds 2 and 3 were isolated for the first time from the stem woods of this plant.