Identification and amino acid sequences of tryptic peptides of a novel ferredoxin-nadp+ oxidoreductase from rice

Abstract

A 37-kDa protein purified from rice thylakoid membranes has been identified as a ferredoxin-NADP+ oxidoreductase based on its catalysis of the reduction of nitro blue tetrazolium via NADPH and its recognition by antibodies against ferrednxin-NADP+ nxidoreduetase. Amino acid sequences determined from tryptic fragments of the enzyme further confirm the identity of the protein and show the presence of unique sequences at the aminoterminus.