Surface Morphology and Biochemical Characteristics of Electrospun Cellulose Nanofibril Reinforced PLA/PBS Hollow Scaffold for Tissue Engineering

Abstract

The treatment of organ failure is one of the primary concerns of healthcare because available organ transplantation techniques have multiple disadvantages. Currently, tissue engineering has emerged as an ingenious approach in which biocompatible and biodegradable scaffolds are used as the alternative to allograft. The PLA/PBS solid electrospun scaffolds have gained a boosted interest in this field. However deficient nutrient diffusion in the implanted area, poor permeability of the seeded cells and insufficient vascularization has been postponing clinical translation of the electrospun scaffolds. In the present research, we have fabricated CNF/PLA/PBS hollow fibrous scaffolds using two different core templates such as mineral oil and glycerol. The physical properties and function were analyzed by using tensile test, scanning electron microscope, contact angle and invitro degradation. The quantity of attached protein was analyzed by using UV absorption and cell proliferation in the scaffold was determined by using MTT reagent kit. On the hollow fiber scaffold the size and distribution of pores affected the wettability. The oil template hollow fiber scaffold showed better cell integration and elevated cell multiplication rate than the glycerol patterned hollow fiber scaffold. Finally, the developed CNF reinforced PLA/PBS hollow fiber scaffold can be useful in tissue regeneration, repair, healing and can also function as wound dressing.