Molecular, cytological and morphological studies on Jassid resistance in cotton (Gossypium hirsutum L.) based on hairiness trait

Abstract

Background: Unravelling the relationship between trichome density and resistance to jassids in upland cotton, nine parental lines, viz. MCU 5, CO 14, CO 17, TCH 1828, KC 2, KC 3, GISV 323, GTHV 15–34, and RHC 1409 were obtained from the Tamilnadu Agricultural University. These genotypes were subjected to molecular analysis using 27 primers, merely the JESPR 154 primer amplifying a 150-bp fragment in genotypes exhibiting the pubescence. Result: This finding validated the association between pubescence and jassid resistance. Further analysis revealed that resistant genotypes (KC 3, GTHV 15–34, GISV 323, and RHC 1409) exhibited significantly higher trichome densities and length compared with susceptible genotypes. These results stalwartly support the hypothesis that trichomes play a pivotal role in conferring resistance to jassids in upland cotton. Conclusion: By breeding cotton varieties with increased trichome density and length, it is possible to reduce jassid infestations, thereby decreasing the reliance on chemical pesticides and promoting a more sustainable agricultural environment.