Sulforaphane increases NRF2 expression and protects alveolar epithelial cells against injury caused by cigarette smoke extract

31Citations
Citations of this article
42Readers
Mendeley users who have this article in their library.

Abstract

Cigarette smoking is a primary risk factor for chronic obstructive pulmonary disease (COPD), as it damages epithelial cells through a variety of mechanisms. Sulforaphane (SFN) is an antioxidant agent, which exerts protective effects against cell damage by activating the nuclear factor erythroid 2 like 2 (NFE2L2; Nrf2). The present study was undertaken to investigate the effects and underlying mechanisms of SFN in preventing cigarette smoke extract (CSE)-induced oxidative damage to RLE-6TN rat lung epithelial cells. MTT assay was used to determine the cytotoxicity of SFN and CSE. The effect of SFN and CSE on cell cycle progression, apoptosis and intracellular reactive oxygen species (ROS) levels were analyzed using flow cytometry. Reverse transcription-quantitative polymerase chain reaction and western blotting were used to quantify mRNA and protein expression levels of Nrf2 respectively. SFN protected RLE-6TN cells from oxidative damage, potentially via increasing Nrf2 expression and reducing ROS levels. In addition, SFN attenuated G1 phase cell cycle arrest and abrogated apoptosis. Therefore, SFN protected alveolar epithelial cells against CSE-induced oxidative injury by upregulating Nrf2 expression. The results of the present study may provide theoretical support for the clinical use of SFN in patients with COPD.

Cite

CITATION STYLE

APA

Jiao, Z., Chang, J., Li, J., Nie, D., Cui, H., & Guo, D. (2017). Sulforaphane increases NRF2 expression and protects alveolar epithelial cells against injury caused by cigarette smoke extract. Molecular Medicine Reports, 16(2), 1241–1247. https://doi.org/10.3892/mmr.2017.6700

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free