Microsatellite based genetic diversity estimation in Kajali sheep and its phylogenetic relationship with other indigenous sheep breeds

Abstract

Microsatellite based characterization of Kajali sheep population found in Punjab and adjoining regions was carried out and genetic diversity measures were analysed. High measures of allele (7.778±0.80) and gene diversity (0.66±0.03) were observed across this population. A significant positive FIS (0.23±0.03) value suggested a deficiency in the number of heterozygotes in Kajali sheep which may be attributed to population sub-structuring into different colour variants (White and Kali Kajali). The population revealed presence of genetic diversity and there was no significant heterozygosity excess indicating the absence of genetic bottleneck in the recent past. The phylogenetic study analysis with 18 other Indian sheep breeds revealed that Kajali sheep clustered in same node with Munjal sheep (bootstrap value of 22%). However, Kajali and Munjal sheep are phenotypically distinct from each other. The genetic characterization of Kajali sheep will help in devising suitable strategies for its genetic improvement, management and recognition at national level.