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Recombinase-mediated cassette exchange provides a versatile platform for gene targeting: Knockout of miR-31b

Genetics (2009) 183(1) 399-402
DOI: 10.1534/genetics.109.105213
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Abstract

A series of vectors has been designed to enhance the versatility of targeted homologous recombination. Recombinase-mediated cassette exchange permits sequential targeting at any locus and improves flexibility in making user-defined mutations. Application of RMCE to delete an intronic microRNA gene is described. Copyright © 2009 by the Genetics Society of America.

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Ruifen, W., Chen, Y. W., Bushati, N., Cliffe, A., & Cohen, S. M. (2009). Recombinase-mediated cassette exchange provides a versatile platform for gene targeting: Knockout of miR-31b. Genetics, 183(1), 399–402. https://doi.org/10.1534/genetics.109.105213

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