Isomaltodextrin prevents dss-induced colitis by strengthening tight junctions in mice

Abstract

Tight junction (TJ) integrity is one of the key factors governing intestinal health. Isomaltodextrin (IMD), a highly branched α-glucan, has been characterized as a new type of soluble dietary fiber. Here, we evaluated the efficacy of IMD to improve the barrier function of TJs. Transepithelial electrical resistance (TER) and Lucifer Yellow (LY) leakage were measured after treating Caco-2 cells in vitro with IMD. A mouse model of dextran sulfate sodium (DSS)-induced colitis was used for the in vivo studies. IMD treatment in vitro maintained TER at higher levels and reduced LY leakage significantly. IMD preloading of mice reduced the signs of DSS-induced colitis compared with the Water group. Immunohistochemical and western blotting analyses of the colon in the IMD group revealed enhanced claudin-3 and ZO-1 expression, indicating the role of IMD-induced TJ strengthening in the alleviation of colitis. The ingestion of IMD has beneficial effects on colon health by improving TJ integrity.