Mapping the human Y chromosome by fingerprinting cosmid clones

Abstract

We have used Y-specific cosmid clones in a random fingerprinting approach to build contigs on the human Y chromosome. Clones derived From two libraries have been analyzed. The construction of one library is described here, the second was the Y chromosome-specific library LLOYNCO3 'M' (Lawrence Livermore National laboratory). To date, we have fingerprinted 4430 cosmids: 377 contigs have been constructed containing from 2 to 39 clones. Along with the singletons, we estimate that we have covered 72.5% of the euchomatic portion of the Y chromosome with fingerprinted clones. Sequence tagged sites are being used to anchor cosmids and contigs onto the YAC framework.