Bioethanol Production from Seaweed Gelidium amansii for Separated Hydrolysis and Fermentation (SHF)

Abstract

The seaweed, Gelidium amansii, was fermented to produce bioethanol. Optimal pretreatment condition was determined as 94 mM H 2 SO 4 and 8% (w/v) seaweed slurry at 121 o C for 60 min. The mono sugars of 40.4 g/L with 67% of conversion from total carbohydrate of 60.6 g/L with 80 g dw/ L G. amansii slurry were obtained by thermal acid hydrolysis pretreatment and enzymatic saccharification. G. amansii hyd-rolysate was used as the substrate for ethanol production by Kluyveromyces marxianus KCTC 7150 and Candida tropica-lis KCTC 7212 using 5L fermentor. The ethanol productions by K. marxianus KCTC 7150 and C. tropicalis KCTC 7212 were 17.8 g/L with Y EtOH of 0.48 at 120 h and 19.3 g/L with Y EtOH of 0.50 at 120 h, respectively.