Fatty acid desaturation during chilling acclimation is one of the factors involved in conferring low-temperature tolerance to young tobacco leaves

Abstract

The FAD7 gene, a gene for a chloroplast ω-3 fatty acid desaturase, is responsible for the trienoic fatty acid (TA) formation in leaf tissues. The TA content of the leaf tissue of the 25°C-grown transgenic tobacco (Nicotiana tabacum cv SR1) plants, in which the FAD7gene from Arabidopsis thaliana was overexpressed, increased uniformly by about 10%. Fatty acid unsaturation in all major leaf polar lipid species increased in the 25°C-grown FAD7 transformants but was approximately the same between the control plants and the FAD7 transformants when grown at 15°C. Therefore, the overexpression of the exogenous FAD7 gene leads to the same consequence in the tobacco plants as the low-temperature-induced TA production that may be catalyzed by an endogenous, temperature-regulated chloroplast ω-3 fatty acid desaturase. In the 25°C-grown control plants, the chilling treatment caused symptoms of leaf chlorosis and suppression of leaf growth. The 25°C-grown FAD7 transgenic plants conferred alleviation of these chilling-induced symptoms. A reduction of the chilling injury similar to that of the FAD7 transformants was also observed in the 15°C-preincubated control plants. These results indicate that the increased TA production during chilling acclimation is one of the prerequisites for the normal leaf development at low, nonfreezing temperatures.