Rapid determination of lovastatin in the fermentation broth of Aspergillus terreus using dual-wavelength UV spectrophotometry

Abstract

Context: Lovastatin, a hypocholesterolemic drug, is produced by submerged fermentation of Aspergillus terreus Thom (Trichocomaceae). High performance liquid chromatography is usually used to determine lovastatin in samples of the fermentation broth. However, this method is inconvenient and costly, especially in the context of high-throughput sample analysis. Objective: A direct and simple dual-wavelength ultraviolet spectrophotometric method for quantifying lovastatin in the fermentation broth of A. terreus was developed. Materials and methods: A. terreus Z15-7 was used for all experiments. The liquid fermentation was conducted at 30°C in a rotary shaker at 150rpm for 15d. Silica gel and neutral alumina column chromatography were used for the separation and purification of lovastatin from the fermentation broth. Results: The limits of detection of lovastatin were 0.320μg/ml in the lovastatin standard solution and 0.490μg/ml in the fermentation broth sample and the limits of quantification of lovastatin were 1.265μg/ml in the lovastatin standard solution and 3.955μg/ml in the fermentation broth sample. The amounts of lovastatin in the fermentation broth ranged from 876.614 to 911.967μg/ml, with relative standard deviations from 1.203 to 1.709%. The mean recoveries of lovastatin using silica gel and neutral alumina column chromatography were 84.2±0.82 and 87.2±0.21%, respectively. Discussion and conclusion: Dual-wavelength UV spectrophotometry is a rapid, sensitive, accurate, and convenient method for quantifying lovastatin in fermentation broth. Neutral alumina column chromatography is more efficient than silica gel column chromatography for the purification and determination lovastatin using the developed dual-wavelength UV spectrophotometry method. © 2014 Informa Healthcare USA, Inc.