Biotransformation of D-limonene to carveol by an endophytic fungus Aspergillus flavus IIIMF4012

Abstract

Microbial biotransformation is an efficient process for making high-value natural products in a controlled and environment friendly manner. Bioconversion of low value monoterpenes to high value products using fungi offers an effective strategy for producing desirable products. Efficient conversion of D-limonene, a monoterpene, into carveol is reported in this study by using an endophytic fungus, Aspergillus flavus IIIMF4012, isolated from the root of Rosa damascena. By optimizing different parameters, isolated strain resulted in the hydroxylation of D-limonene to carveol effectively and the process was carried out at 1 L scale which resulted in the formation of a mixture of carveol isomers (cis- carveol 23.82%, trans- carveol 17.21%). Further, the biotransformation was scaled up in 5 L fermenter and the product was characterized by GC-MS and NMR (1H & 13C). As a result, an efficient procedure was established for biotransforming a low-value molecule i.e., D-limonene into a high-value product i.e.,carveol.