Extraction of B-galactosidase Enzyme from Lacticacid Bacteria

Abstract

The dairy industry has employed the B-galactosidase enzyme (EC 3.2.1.23) to reduce lactose intolerance. The ability of probiotic Lactobacillus spp. to produce B-galactosadase enzyme and hydrolyze 5-bromo-4-chloro-3-indolyl-D-galactopyranoside (X-Gal) and ONPG was investigated. Through the use of sontication to lyse the cell, crude enzyme extract was produced. Ortho-nitrophenyl-beta-D-galactopyranoside (ONPG) was used as an enzyme substrate in order to measure the B-galactosidase enzyme activity. B-galactosidase activity is demonstrated by blue-colored colonies of Lactobacillus spp. on X-Gal plates. The ideal temperature and pH for the synthesis of B-galactosidase were pH 7 and 40 °C. People who are lactose intolerant may benefit from Lactobacillus spp., which produces B-galactosidase enzyme as a probiotic for dairy products.