Structural and Functional Dynamics of Secondary Metabolite from Actinokineospora cibodasensis against Pseudomonas aeruginosa Biofilm

Abstract

Biofilm formation has incredible detrimental effects and has brought huge issue to our daily life. Pseudomonas aeruginosa is recognized as an opportunistic pathogen and its one of the antibiotic resistance mechanisms includes biofilm formation. To survive against harsh environmental conditions the microorganisms form an extracellular polymeric substance matrix. Natural products, particularly, marine natural products have been established useful in providing new chemical entities to mark biofilm inhibition and dole out as a rich source of privileged frames for studying chemical as well as biological applications. In the present study, antibiofilm potential of bioextract from Actinokineospora cibodasensis (MW513387) is tested against Pseudomonas aeruginosa (ATCC 27853) biofilm. The analysis of the quorum sensing modulation was done using exopolysaccharide (EPS) quantification and Cytotoxicity assays were performed to check toxicity. Scanning electron microscopy (SEM) was also conducted to visualize the mitigation of biofilm using the biocontrol agent. All the assays conducted evidently indicated that the potential bioactive compound from Actinokineospora cibodasensis has greater inhibition effect against pathogenic biofilm. In the near-term years it is expected that these pharmaceutical compounds from natural source be translated into antibiofilm drugs.