Bioactive Content and Anticancer Bioactivity of MCF-7 Cell in Smadan Root Extract from Bitung City Forest North Sulawesi

Abstract

Cancer is a disease with the second largest death rate in the world. Cancer Prevalence has continued to increase in the last decades. The research was conducted from May to August 2023. The aim of this research was to determine the bioactive content and anticancer bioactivity of michigan cancer foundation-7 (MCF-7) in Smadan root extract from the Bitung city forest, North Sulawesi. The bioactive content was analyzed using the high performance liquid chromatography (HPLC) method, total flavonoids were determined using ultraviolet and visible light spectrophotometry, while the anticancer activity was tested in vitro on MCF-7 cell cancer (breast cancer cells). The results of the high performance liquid chromatography analysis research showed that 26 compounds were at a wavelength of 254 nanometer, while 37 compounds were at a wavelength of 310 nanometer. The total flavonoid content of dry Smadan root extract was 62.66 mg QE/g, while the wet extract was 14 mg QE/g and the cytotoxic activity of Smadan root extract had IC50 = 50.12 μg/mL. These results indicate that Smadan root extract has strong cytotoxicity. Thus, the ethanol extract of Smadan roots has the potential to be developed as a source bioactives and drug of anticancer that can help people to cure cancer.