Microbial conversion of rare ginsenoside Rf to 20(S)-protopanaxatriol by Aspergillus niger

Abstract

In this study, rare ginsenoside Rf was transformed into 20(S)-protopanaxatriol (PPT(S)) by glycosidase from Aspergillus niger. By investing the reaction conditions, the optimal conditions were obtained, as follows: pH 5.0, temperature 55°C, and substrate concentration 1.25 mmol/l. Under optimal conditions, PPT(S) (1.13 μmol) prepared from 1.25 μmol Rf showed a higher yield (90.4%). The enzymatic reaction was analyzed by reversed-phase HPLC, suggesting the transformation pathway: Rf → Rh1(S) → PPT(S).