Evaluation of preliminary anti-microbial efficacy of curcumin and chitosan nanoparticles with and without photoactivation against candida albicans – An invitro study

Abstract

Context: The success of endodontic treatment lies in adequate debridement of the root canal. There are various microorganisms responsible for the failure of root canal treatment, one among them is Candida albicans. Sodium hypochlorite is considered as the gold standard. Since it has various disadvantages newer irrigants like Curcumin and Chitosan nanoparticles have been tried. Aim: The aim of this study was to evaluate the preliminary anti-microbial efficacy of Curcumin and Chitosan nanoparticles with and without photoactivation against Candida albicans. Materials and Methods: The antimicrobial activity of Curcumin, Chitosan nanoparticles with and without Photoactivation, Saline and Sodium hypochlorite was assessed. MIC was done with serial dilution method, incubated for 48-72 hours andobserved for turbidity.MBC was done from the MIC dilutions tubes, first 3 tubes were plated, incubated for 24 hrs and the colony was counted. For Time kill assay broth with the compound was mixed, plated and inoculated at 1hr, 2hrs and 4hrsinterval and colony count was noted. Statistical analysis: Results were tabulated and statistical analysis was done using Analysis of Variance (ANOVA) and Tukey multiple comparison test. Results: Photoactivated Curcumin has shown the best antimicrobial results as compared to Curcumin, Chitosan nanoparticles (photoactivated and nonphotoactivated) and 3% Sodium hypochlorite. Conclusion: Curcumin with and without photo activation and Chitosan nanoparticles can be considered as an alternate endodontic irrigants against sodium hypochlorite.