High intake of green tea decreased hemoglobin and hematocrit levels in Rattus novergicus strain wistar albino

Abstract

Background: Anemia is one of four nutritional problems in Indonesia with a prevalence of 21.7%, the type of anemia is iron deficiency anemia. One of the causes of iron by tannins present in green tea. The purpose of this study is to determine the effect of green tea on hemoglobin and hematocrit amount in Rattus novergicus strain wistar albino.Methods: This research used experiment with post-test only control group design. Twenty four rats were divided into four groups: the control group and the treatment group were given a different dose of 5.6g in a 1.8ml/200gr solvent, 8.4g in a 1.8ml/200g solvent, 11.2g in 1.8ml/200gr solvent for a month. Blood is taken through 2 ml orbital sinus using capillary pipette. hemoglobin examination using spectrophotometer and hematocrit checking using microhematocrit tool. The study was conducted in pharmaceutical laboratories and biochemical laboratories. Data analysis using one way ANOVA and Kruskal Wallis with degree of significance p<0.05.Results: The results of the study were found the mean differences amount of hemoglobin and hematocrit. The mean of control group in hemoglobin content was 16.39gr%, in treatment group were P1: 13.64gr%, P2: 11.19gr% and P3: 10.49 gr%, while in hematocrit control group was 44.83%, in treatment group were P1: 44.83%, P2: 39.17% and P3: 37.17%. Result of the test using one way ANOVA, which found the effect of green tea on hemoglobin (p value = 0.000) and result of the test using Kruskal-Wallis, which found the effect of green tea on hematocrit (p value = 0.017).Conclusions: Based on the results of this study concluded that there is influence of green tea on the decrease of hemoglobin and hematocrit amount in Rattus novergicus strain wistar albino. It is suggested to pay more attention to dose of green tea with normal limit of 8.4gr.